Heart rate reserve at ventilatory thresholds, maximal lactate steady state and maximal aerobic power in well-trained cyclists: training application


  • Ricardo Morán-Navarro Human Performance and Sports Science Laboratory, University of Murcia, Spain.
  • Ricardo Mora-Rodríguez Exercise Physiology Laboratory, University of Castilla-La Mancha, Toledo, Spain
  • Víctor Rodríguez-Rielves Human Performance and Sports Science Laboratory, University of Murcia, Spain.
  • Paulo De la Fuente-Pérez Human Performance and Sports Science Laboratory, University of Murcia, Spain.
  • Jesús G. Pallarés Human Performance and Sports Science Laboratory, University of Murcia, Spain.


Introduction: Several physiological tests have been developed to predict cycling performance. However, the high costs and expertise needs to perform these tests detracts coaches and athletes from using them habitually. The aim of this study is to provide the equivalence between these physiological assessments and heart rate reserve (HRR) to facilitate training advice to cyclists. Materials and Methods: Thirty three aerobically-trained male cyclists ( O2max 62.1±4.6 ml·kg-1·min-1) performed two graded exercise tests (GXT; 50 W warm-up followed by 25 W·min-1) to exhaustion. O2 and CO2 data were collected throughout GXT and several continuous tests were performed to detect maximal lactate steady state workload (MLSS). Results: VT1, VT2 and O2max were achieved at power outputs of 184±36, 298±36 and 390±34 W, respectively corresponding with 66±9, 88±6 and 100% of HRR. MLSS (n=14), occurred at 256±31 W. These HRR defined five training zones; 53-62% HRR (zone R0), 62-71% HRR (zone R1), 74-86% HRR (zone R2), 86-99% HRR (zone R3) and 100% HRR (zone R3+). Discussion: We found the HRR correspondence to ventilatory aerobic and anaerobic thresholds (i.e., VT1 and VT2) MLSS and O2max. Those HRR defined 5 distinguishable training zones corresponding to those physiological events that could be used for optimizing training.


Amann, M., Subudhi, A.W., & Foster, C. (2006). Predictive validity of ventilatory and lactate thresholds for cycling time trial performance. Scandinavian Journal of Medicine & Science in Sports, 16, 27–34.

American College of Sports Medicine (2013). ACSM’s guidelines for exercise testing and prescription. Lippincott Williams & Wilkins.

Arts, F.J. Kuipers, H. (1994). The relation between power output, oxygen uptake and heart rate in male athletes. International Journal of Sports Medicine, 15, 228–231.

Aunola, S., Rusko, H. (1984). Reproducibility of aerobic and anaerobic thresholds in 20–50 year old men. European Journal of Applied Physiology and Occupational Physiology, 53(3), 260-266.

Beaver, W.L., Wasserman, K., & Whipp, B.J. (1986). A new method for detecting anaerobic threshold by gas exchange. Journal of Applied Physiology, 60, 2020–2027.

Beneke, R. (1995). Anaerobic threshold, individual anaerobic threshold, and maximal lactate steady state in rowing. Medicine and science in sports and exercise, 27(6), 863.

Beneke, R., von Duvillard, S.P. (1996). Determination of maximal lactate steady state response in selected sports events. Medicine Science & Sports Exercise, 28, 241–246.

Borg, G. (1998). Borg's perceived exertion and pain scales. Human kinetics.

Bulbulian, R., Wilcox, A.R., Darabos, B.L. (1986). Anaerobic contribution to distance running performance of trained cross-country runners. Medicine & Science in Sports & Exercise, 18, 107-113.

Burgomaster, K.A., Howarth, K.R., Phillips, S.M., Rakobowchuk, M., Macdonald, M.J., McGee, S.L. & Gibala, M.J. (2008). Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. Journal of Physiology, 586, 151–160.

Burgomaster, K.A., Hughes, S.C., Heigenhauser, G.J., Bradwell, S.N. & Gibala, M.J. (2005). Six sessions of sprint interval training increases muscle oxidative potential and cycle endurance capacity in humans. Journal of Applied Physiology, 98, 1985–1990.

Lounana, J., Campion, F., Noakes, T. D., & Medelli, J. (2007). Relationship between% HRmax,% HR reserve,% VO2max, and% VO2 reserve in elite cyclists. Medicine & Science in Sports & Exercise, 39(2), 350-357.

Cavanagh, P.R., Kram, R. (1985). The efficiency of human movement - a statement of the problem. Medicine & Science in Sports & Exercise, 17, 304-308.

Copp, S. W., Hirai, D. M., Musch, T. I., & Poole, D.C. (2010). Critical speed in the rat: implications for hindlimb muscle blood flow distribution and fibre recruitment. The Journal of Physiology, 588(24), 5077-5087.

Del Coso, J., Hamouti, N., Aguado-Jimenez, R., Mora-Rodriguez, R. (2009). Respiratory compensation and blood pH regulation during variable intensity exercise in trained versus untrained subjects. European Journal of Applied Physiology, 107, 83–93.

Docherty, D., Sporer, B. (2000). A proposed model for examining the interference phenomenon between concurrent aerobic and strength training. Sports Medicine, 30(6): 385-94

Esteve-Lanao, J., Foster, C., Seiler, S., & Lucia, A. (2007). Impact of training intensity distribution on performance in endurance athletes. The Journal of Strength and Conditining Research, 21(3), 943-949.

Farrell, P.A., Wilmore, J.H., Coyle, E.F., Billing, J.E., & Costill, D.L. (1979). Plasma lactate accumulation and distance running performance. Medicine & Science in Sports & Exercise, 11(4), 338-44.

Foster, C. (1983). VO2max and training indices as determinants of competitive running performance. Journal of Sports Sciences, 1, 13–22.

García-Pallarés, J., & Izquierdo, M. (2011). Strategies to optimize concurrent training of strength and aerobic fitness for rowing and canoeing. Sports Medicine, 41(4), 329-343.

García-Pallarés, J., Sánchez-Medina, L., Carrasco, L., Díaz, A., & Izquierdo, M. (2009). Endurance and neuromuscular changes in world-class level kayakers during a periodized training cycle. European Journal of Applied Physiology, 106(4), 629-638.

García-Pallarés, J., Sánchez-Medina, L., Pérez, C.E., Izquierdo-Gabarren, M., & Izquierdo, M. (2010). Physiological effects of tapering and detraining in world-class kayakers. Medicine & Science in Sports & Exercise, 42(6), 1209.

Gaskill, S.E., Ruby, B.C., Walker, A.J., Sanchez, O.A., Serfass, R.C., Leon, A.S. (2001). Validity and reliability of combining three methods to determine ventilatory threshold. Medicine & Science in Sports & Exercise, 33(11), 1841-1848.

Gibala, M.J., Little, J.P., MacDonald, M.J., & Hawley, J.A. (2012). Physiological adaptations to low‐volume, high‐intensity interval training in health and disease. The Journal of Physiology, 590(5), 1077-1084.

Gibala, M.J., Little, J.P., van Essen, M., Wilkin, G.P., Burgomaster, K.A., Safdar, A., Raha, S. & Tarnopolsky, M.A. (2006). Short-term sprint interval versus traditional endurance training: similar initial adaptations in human skeletal muscle and exercise performance. Journal of Physiology, 575, 901–911.

Helgerud, J., Hoydal, K., Wang, E., Karlsen, T., Berg, P., Bjerkaas, M., & Hoff, J. (2007). Aerobic High-Intensity Intervals Improve VO2max More Than Moderate Training. Medicine and Science in Sports and Exercise, 39(4), 665.

Jones, A.M., Wilkerson, D.P., DiMenna, F., Fulford, J., & Poole, D.C. (2008). Muscle metabolic responses to exercise above and below the “critical power” assessed using 31P-MRS. American Journal of Physiology-Regulatory: Integrative and Comparative Physiology, 294(2), 585-593.

Lucía, A., Hoyos, J., Pérez, M., Chicharro, J.L. (2000). Heart rate and performance parameters in elite cyclists: a longitudinal study. Medicine and Science in Sports and Exercise, 32, 1777–1782.

Lucía, A., Sánchez, O., Carvajal, A., Chicharro, J.L. (1999). Analysis of the aerobic-anaerobic transition in elite cyclists during incremental exercise with the use of electromyography. British Journal of Sports Medicine, 33, 178–185.

Lucia, A., Hoyos, J., Carvajal, A., & Chicharro, J.L. (1999). Heart rate response to professional road cycling: the Tour de France. International Journal of Sports Medicine, 20(3), 167-172.

MacDougall, D., Sale, D. (1981). Continuous vs interval training: a review for the athlete and the coach. Canadian Journal of Applied Sport Sciences, 6(2): 93-7

Mora-Rodríguez, R., Pallarés, J.G., López-Gullón, J.M., López-Samanes, Á., Fernández-Elías, V.E., Ortega, J.F. (2015). Improvements on neuromuscular performance with caffeine ingestion depend on the time-of-day. Journal of Science and Medicine in Sport, 18(3), 338-342.

Mujika, I., Padilla, S. (2001). Physiological and performance characteristics of male professional road cyclists. Sports Medicine, 31(7), 479-487.

Noakes, T. (1988). Implications of exercise testing por prediction of athletics performance: a contemporary perspective. Medicine & Science in Sports & Exercise, 20, 319-330.

Paavolainen, L., Nummela, A., Rusko, H. (2000). Muscle power factors and VO2max as determinants of horizontal and uphill running performance. Scandinavian Journal of Medicine & Science in Sports, 10, 286-291.

Paavolainen, L., Häkkinen, K., Hämäläinen, I., Nummela, A., Rusko, H. (1999) Explosive-strength training improves 5-Km running time by improving running economy and muscle power. Journal of Applied Physiology, 86, 1527-1533.

Pallarés et al., 2016. Validity and reliability of ventilatory and blood lactate thresholds in well-trained cyclist. PLoS One. In press.

Pfitzinger, P., Freedson, P.S. (1998). The reliability of lactate measurements during exercise. International Journal of Sports Medicine, 19, 349–357.

Prud'Homme, D., Bouchard, C., Leblance, C., Landry, F., Lortie, G., Boulay, M.R. (1984). Reliability of assessments of ventilatory thresholds. Journal of Sports Sciences, 2(1), 13-24.

Rakobowchuk, M., Tanguay, S., Burgomaster, K.A., Howarth, K.R., Gibala, M.J. & MacDonald, M.J. (2008). Sprint interval and traditional endurance training induce similar improvements in peripheral arterial stiffness and flow-mediated dilation in healthy humans. American Journal of Physiology Regulatory, Integrative and Comparative

Physiology, 295, 236–242.

Seiler, S., Tønnessen, E. (2009). Intervals, thresholds, and long slow distance: the role of intensity and duration in endurance training. Sportscience, 13, 32-53.

Skinner, J.S., McLellan, T.H. (1980). The transition from aerobic to anaerobic metabolism. Research Quarterly for Exercise and Sport, 51, 234–248.

Wasserman, K., Whipp, B.J., Koyl, S.N., Beaver, W.L. (1973). Anaerobic threshold and respiratory gas exchange during exercise. Journal of Applied Physiology, 35, 236–243.

Weltman, A., Snead, D., Stein, P., Seip, R., Schurrer, R., Rutt, R., & Weltman, J. (1990). Reliability and validity of a continuous incremental treadmill protocol for the determination of lactate threshold, fixed blood lactate concentrations, and VO2max. International Journal of Sports Medicine, 11(1), 26-32.

Weston, S.B., Gabbett, T.J. (2001). Reproducibility of ventilation of thresholds in trained cyclists during ramp cycle exercise. Journal of Science and Medicine in Sport, 4, 357–366.






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